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Next-Generation-Sequencing

Next-Generation-Sequencing (NGS) is a high-throughput method for massive, parallel sequencing of millions of nucleotide fragments. A NGS experiments needs a carefully evaluated study design, good quality source material (DNA or RNA), a so-called library preparation, the sequencing run to generate big datasets and bioinformatic / statistician expertise to extract meaningful biological results from these data.
In our institute, we offer to do the library preparation process as well as the NGS sequencing on the basis of a scientific collaboration. Currently, the fleet of NGS sequencing devices in our institute comprises two Illumina NovaSeq 6000, two HiSeq 3000/4000, one NextSeq 500, two MiSeq systems and a PacBio Sequel.

Sequencing Instruments @CCGA

Illumina MiSeq

The Illumina MiSeq is a small benchtop sequencer with a maximum output of 50 M paired-end reads and 15 Gigabases. Due to its low throughput and rapid turnaround time, the MiSeq is recommended for targeted re-sequencing (small gene panels, e.g. BRCA), amplicon sequencing (e.g. 16S) and sequencing of small genomes (e.g. bacteria).
See output specifications of the MiSeq here.

Illumina NextSeq 500

The Illumina NextSeq is a small high-throughput benchtop sequencer with a maximum output of 800 M paired-end reads and 120 Gigabases of data. With the choice of a variety of different Flowcells, the NextSeq is a highly flexible device and can be used for Exome sequencing, transcriptome sequencing and Panel-Sequencing. Because of its rapid turnaround time, we recommend this device for urgent samples.
See output specifications of the NextSeq here.

Illumina HiSeq 3000 / 4000

With up to 625 M reads and 750 Gigabases output per Flowcell the Illumina HiSeq 3000 / 4000 is a high-throughput Sequencer. The Flowcell has 8 lanes that can be loaded individually. The HiSeq is recommended for high-throughput experiments, e.g. exomes, genomes and transcriptomes. Because a HiSeq Flowcell is usually filled by projects of several users, the turnaround time of your order is dependant on other projects filling unused lanes.
See output specification of the HiSeq 3000 / 4000 here.

Illumina NovaSeq 6000

The NovaSeq 6000 is our newest addition to our sequencer lineup. With a large variety of different Flowcells (S1, S2, S4) offering different read-length and data outputs, the NovaSeq is highly flexible device. With up to 10 billion reads and 3000 gigabases output the NovaSeq is the sequencer with the highest data output available. The NovaSeq is recommended for high-throughput experiments, e.g. exomes, genomes and transcriptomes. 4 lanes can be loaded individually. Note that for the NovaSeq, no single-end sequencing option is available.
See output specifications of the NovaSeq 6000 here.

PacBio Sequel

The PacBio Sequel is a long-read sequencer of the third generation, producing up to 500.000 reads with read-lengths up to 30 kilobases. With the circular-consensus-sequencing technology, read accuracies of 99.99 % can be achieved. The Sequel is recommended for de novo genome projects and structural variant detection as well as full length transcript RNAseq (IsoSeq). In addition, multiplexed long amplicon sequencing (e.g. full lengths 16S) is possible.
See specifications of the Sequel here and information on the special requirements of third generation sequencers here.

News

Oct. 26, 2020
Follow us on Twitter @CcgaKiel ...

Sep. 09, 2020
Turn around time momentarily increased: 12 weeks ...

Mar. 30, 2020
3rd DFG Sequencing Call: new deadline 9.9.2020 ...

Mar. 16, 2020
Limited service due to Corona ...

Dec. 12, 2019
Christmas break 19.12.2019 - 06.01.2020 ...

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